What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water and the resulting solution is brought to a final volume of 0.50 mL with water what is the final dilution of the original sample? 109 10 8. A technologist mixes 100 uL of patient sample with 300 UL of diluents and multiplies the patient result by 3 to correct for the dilution factor. Has the tech multiplied by the correct dilution factor? If not then whats the correct dilution factor? 9. If the beginning tube dilution is 1:40 how many tubes are required in a serial dilution to achieve a sample dilution of 1:1280? ng sample concentrations based upon given dilutions. Final (Diluted) ation 1.8 mL a. 10 (1:10) b. 0.50 mL 0.10 mL 0.01 mL 0.02 mL 4.50 mL 0.40 mL C. d. 25 50 2. When using 100 uL of patient sample how much saline (diluent) is needed to create a 1:6 dilution? 3. 400 uL of serum is needed to make a 1:4 dilution. How much buffer is necessary to make this dilution? Convert your answer into mL. 4. Convert the following: a 250 uL to mL b) 0.05 mL to uL d) 100 uL to mL c) 0.5 mL to uL 5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT. Patient Sample (ul) 20 UL Diluent (mL) 0.18 mL Dilution Factor 10 (1:10) 1.2
